J. Shah, A. Poruri, O. Atto 550 for fluorescence, ≥90% (HPLC) | Sigma-Aldrich P. Damke, L. Celma, S. Kondekar, A. Sitemap, ISO 9001:2015 Claude, A. Moreau, J. Lumeau, J. Wenger, Extending Single-Molecule Förster Resonance Energy Transfer (FRET) Range beyond 10 Nanometers in Zero-Mode Waveguides, ACS Nano 13, 8469 (2019). atto 550 flow cytometry channel. Douglas, L.A. Gardner, M.C. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers. ✓ Adapting the website to color blind people DNA Barcoded Antibodies for PhenoCycler-Fusion | Leinco A (-) in a table cell represents no applicable spillover. J. Nikolic, L. Belot, H. Raux, P. Legrand, Y. Gaudin, A. Albertini, Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein, Nature Communications 9, 1029 (2018). Atto 550 is a fluorescent compound with an excitation peak at 554 nm and an emission peak at 575 nm. To add one or more spectra to the graph, click either "fluorophore" in the "Add" submenu on the left or click the center of the empty display panel - a menu will appear at the bottom of the page. Under the "Export" submenu on the left side of the screen, the user may instantly download the spectrum as a .png file to their computer by clicking "Image" under the "Export" Menu on the left side of the window. (d) Overlay of the three components. 0000238713 00000 n Maximally excitable by the 488 nm laser and emitting at 580 nm, this dye is brighter than Alexa Fluor 532 and as bright as PE from the 488 nm laser, without the 561 nm excitation, making it an excellent choice for use in multicolor panel building. Approximately 10,000 cells can be analyzed and processed by a computer in less than one minute. 0000190721 00000 n Northland College Women's Hockey Roster, %PDF-1.4 % Flow cytometry combines the flexibility and sensitivity of fluorescence technology with high speed and data integration capabilities. Not for use in diagnostic procedures. D. Rutz, Q. Luo, L. Freiburger, T. Madl, V. Kaila, M. Sattler, J. Buchner, A switch point in the molecular chaperone Hsp90 responding to client interaction, Nature Communications 9, 1472 (2018). Grüning et al., A molecular toolkit for population genetic investigations of the ash dieback pathogen Hymenoscyphus pseudoalbidus, For. ATTO 594 exhibits excellent water solubility, very good stability over . Mark, U. Khadilka, F. Mohring, R. Moon, R. Ramasamy. Changing color contrast based on dark backgrounds Rua Almada Negreiros Lote 5, Loja 14 2615-275 Alverca do Ribatejo - Portugal Tel. JZUDuc^lH(6s MlN.S&~n^cjmC&F Aq,6K7J J* K TlM\%p.%z dk1fPRNWFW@cAX+xWV~ gL1x0Gbj>ZBr a].#C ]uyWV(0zEI t+)rl@;X/V])'m&FU i ATTO 550 is a yellow emitting dye that can be excited by the 532 laser and emission captured by the 585/42 filter. M. Chai, S. Razavi Bazaz, R. Daiyan, A. Razmjou, M. Ebrahimi Warkiani, R. Amal, V. Chen, Biocatalytic micromixer coated with enzyme-MOF thin film for CO2 conversion to formic acid, Chemical Engineering Journal 426, 130856 (2021). Le Marois, K. Suhling, D. Richards, A. Zayats. BioSyst. I. Hoffecker, S. Chen, A. Gådin, A. Bosco, A. Teixeira, B. Högberg, Solution‐Controlled Conformational Switching of an Anchored Wireframe DNA Nanostructure, Small 15 (2019). Shipping costs, Terms and Conditions Sumita, M.R. 0000186798 00000 n Y. Jiang, A. Matevossian, H.-S. Huang, J. Straubhaar, Sch. Two of the most commonly used fluorophores in flow cytometry, R-phycoerythrin (R-PE) and allophycocyanin (APC), are phycobiliproteins originally derived from algae. Yang, E. Cooper, B. Chen, K. Siminovitch, A. Peterson. (2009). This website is run by the accessibility program of the "Accessible with a Click" company and is run via a designated accessibility server. M. Chinnaraj, D. Barrios, C. Frieden, T. Heyduk, R. Flaumenhaft, N. Pozzi, Bioorthogonal Chemistry Enables Single-Molecule FRET Measurements of Catalytically Active Protein Disulfide Isomerase, Encyclopedia of Analytical Chemistry 22, 134 (2021). The fluorescence is excited most efficiently in the 580 – 615 nm range. U. Chio, S. Chung, S. Weiss, S.-O. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 . L. Dirix, K. Kennes, E. Fron, Z. Debyser, M. van der Auweraer, J. Hofkens, S. Rocha. A. Andreoni, L. Nardo, R. Rigler, Time-resolved homo-FRET studies of biotin-streptavidin complexes, Journal of Photochemistry and Photobiology B: Biology 162, 656 (2016). M. Pazos, K. Peters, M. Casanova, P. Palacios, M. VanNieuwenhze, E. Breukink, M. Vicente, W. Vollmer. If a selected compound is no longer of interest, click the checkbox to the right of the compound name as displayed on the bottom of the page to deselect it. PDF Product Information: ATTO 550 ATTO-633. J. Wardyn, A. Chan, A. Jeyasekharan, A Robust Protocol for CRISPR-Cas9 Gene Editing in Human Suspension Cell Lines, Current Protocols 1, e286 (2021). Syeda Rubaiya Nasrin, Tsukasa Ishihara, Arif Md. M. Urban, S. Both, C. Zhou, A. Kuzyk, K. Lindfors, T. Weiss, N. Liu, Gold nanocrystal-mediated sliding of doublet DNA origami filaments, Nature Communications 9, 1454 (2018). Western blot analysis (unlabeled antibody, Subscribe Newsletters and Email Updates, STIM1 (extracellular) Blocking Peptide (#BLP-CC063), Anti-CRACR2A (EFCAB4B) Antibody (#ACC-324), Anti-Human Orai1 (extracellular) Antibody (#ACC-060), Anti-Human Orai1 (extracellular)-FITC Antibody (#ACC-060-F), Anti-Orai1 (extracellular) Antibody (#ACC-062), Anti-Orai1 (extracellular)-ATTO Fluor-488 Antibody (#ACC-062-AG), Anti-SLC35G1 (extracellular) Antibody (#ANT-196), Anti-STIM1 (extracellular) Antibody (#ACC-063), Anti-TMEM66-ATTO Fluor-594 Antibody (#ACC-067-AR), Immunohistochemistry (IHC) Protocols for Paraffin-embedded Sections, Immunocytochemistry (ICC) Protocols for Fixed or Live Cells: Indirect and Direct Methods. Protect from light. K.A.M. Maximum absorption 601 nm; Maximum fluorescence 627 nm. A fluorophore free in solution may have a different quantum yield than the same fluorophore attached to a protein, which in turn also depends on the extent of protein-to-fluorophore labeling (1-3). G. Lin, M. Lewandowska, Plasmon-enhanced fluorescence provided by silver nanoprisms for sensitive detection of sulfide, Sensors and Actuators B: Chemical 292, 241 (2019). atto 550 flow cytometry channel - carolinapoolservices.com J. de Torres, M. Mivelle, S. Moparthi, H. Rigneault, N. van Hulst, M. Garcia-Parajo, E. Margeat, J. Wenger, Plasmonic Nanoantennas Enable Forbidden Forster Dipole-Dipole Energy Transfer and Enhance the FRET Efficiency, Nano letters 16, 6222 (2016). O. Afolabi, A. Roeder, A. Iyengar, S. Hadi, >Evaluation of genetic markers for forensic identification of human body fluids>, Forensic Science International: Genetics Supplement Series 6, e241-e243 (2017). As supplied ATTO 550 consists of three isomers with identical absorption and fluorescence. V. Mller, F. Westerlund, Optical DNA mapping in nanofluidic devices, Lab on a chip 17, 579 (2017). Designed to be affordable and expandable, the BD LSRFortessa System has the flexibility to support the expanding needs of multicolor flow cytometry assays. Terms of Payment. Lo, F. Emran, I. Kays, X.-J. Products for Flow Cytometry CF Dyes for Flow Cytometer Laser Lines 2 More photostable and less spill over in the 525/50 green channel than Pacific ATTO 550, Cy3, DyLight 549, TRITC Brighter than Cy3 Comparable to Alexa Fluor 555 Green (532 nm). Despite our efforts to enable website browsing for all the website pages, there may be website pages that haven't been made accessible yet or may lack a suitable technical solution. This label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. X. Chen, T. Liu, X. Qin, Q. Nguyen, S. Lee, C. Lee, Y. Ren, J. Chu, G. Zhu, T.-Y. 4, 1000134 (2013). P. Petazzi, L. Miquel-Serra, S. Huertas, C. González, N. Boto, E. Muñiz-Diaz, P. Menéndez, A. Sevilla, N. Nogués, ABO gene editing for the conversion of blood type A to universal type O in Rhnull donor-derived human-induced pluripotent stem cells, Clinical and Translational Medicine 12, e1063 (2022). 0000007840 00000 n S. Lee, J.-H. Bong, J. Jung, J. R. Masoud, R. Tsukanov et al., Studying the Structural Dynamics of Bipedal DNA Motors with Single-Molecule Fluorescence Spectroscopy, ACS Nano 6, 6272 (2012). 11, 085602 (2014). A ribonucleoprotein transfection strategy for ... - Cell & Bioscience Xie, J. Guillot, S. Yucel, P. Li, J. Galván, E. Karamitopoulou, I. Zlobec, D. Ataca, F. Gallean, P. Zhang, J. Rodriguez-Calero, M. Rubin, M. Tichet, K. Homicsko, D. Hanahan. Antibody concentration after reconstitution. Phone: 305-822-0666 14, 4707 (2014). Two levels of system alignment are . 0000032428 00000 n A one parameter histogram plotting channel number vs. number of events. Janelia Fluor® 549 Yellow-Green 549 nm Yellow 571 nm Bright DyLight 550 Yellow-Green 562 nm Yellow 576 nm Bright PE Yellow-Green 496 nm, 565 nm Yellow 578 nm Bright . Rashedul Kabir, Akihiko Konagaya, Kazuki Sada, Akira Kakugo, Comparison of microtubules stabilized with the anticancer drugs cevipabulin and paclitaxel, Polymer Journal 52, 969 (2020). M. Malle, P. Löffler, S. Bohr, M. Sletfjerding, N. Risgaard, S. Jensen, M. Zhang, P. Hedegård, S. Vogel, N. Hatzakis, Single-particle combinatorial multiplexed liposome fusion mediated by DNA, Nature Chemistry 14, 558 (2022). W. Ren, S. Wen, S. Tawfik, Q. Su, G. Lin, L. Ju, M. Ford, H. Ghodke, A. van Oijen, D. Jin. Park, I. Jeon, B. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta™. The program allows the website to follow the guidelines for internet content accessibility WCAG 2.0 to level AA. Atto 550 is spectrally similar to. Atto 550 is an alternative to rhodamine dyes, Cy3, and Alexa Fluor 550, offering more intense brightness and increased photostability. B. Dumontel, F. Susa, T. Limongi, V. Vighetto, D. Debellis, M. Canta, V. Cauda. The CD4+CD25+(high) gating strategy shown here was used to identify Treg populations. W. Ren, S. Wen, S. Tawfik, Q. Su, G. Lin, L. Ju, M. Ford, H. Ghodke, A. van Oijen, D. Jin, Anisotropic functionalization of upconversion nanoparticles, Chemical Science 9, 4352 (2018). Long, K. Ubych, E. Jagu, R. Neely, FRET-Based Method for Direct, Real-Time Measurement of DNA Methyltransferase Activity, Bioconjugate Chemistry 32, 192 (2021). Thermo Fisher Scientific. Fan. Maximum absorption 501 nm; maximum fluorescence 523 nm. -ATTO 550 product information, - NHS-ester Akbarian, Isolation of neuronal chromatin from brain tissue, Neuroscience 9, 42 (2009). M. Jahn, A. Rehn et al., The charged linker of the molecular chaperone Hsp90 modulates domain contacts and biological function, PNAS 111, 17881 (2014). T. Munmun, A. Kabir, K. Sada, A. Kakugo, Complete, rapid and reversible regulation of the motility of a nano-biomolecular machine using an osmolyte trimethylamine-N-oxide, Sensors and Actuators B: Chemical 304, 127231 (2020). When phycobiliproteins are exposed to organic . Click "Hide Crosshairs" to return to the default. Maximum absorption 593 nm; Maximum fluorescence 622 nm. J. Shah, H. Weltman, P. Narciso, C. Murphy, A. Poruri, S. Baliga, L. Sharon, M. York, G. Cunningham, S. Miller, L. Caviedes, R. Gilman, E. Desmond, R. Ramasamy, Dual color fluorescence in situ hybridization (FISH) assays for detecting Mycobacterium tuberculosis and Mycobacterium avium complexes and related pathogens in cultures, PLOS ONE 12, e0174989 (2017). Y. Subscribe Newsletters and Email Updates. Technical Support, Order Information Chem. Kaminski et al., Light-inducible molecular beacons for spatiotemporally highly defined activation, Chem. T. Thumberger, T. Tavhelidse-suck, J. Gutierrez-triana, A. Cornean, R. Medert, B. Welz, M. Freichel, J. Wittbrodt. C. Kim, O.-c. Lee, J.-Y. Miller, R. Vogel, P.P.T. Chem. The high quality barcoded antibodies enable a focus on multiplex imaging and data analysis. Surawsky, S.R. She, R. Tornay, E. Leimgruber, D. Bernasconi, L. Lagopoulos, P. Renaud, N. Demierre, P. van den Bogaard, Rapid, sensitive and real-time multiplexing platform for the analysis of protein and nucleic-acid biomarkers, Analytical Chemistry 87, 1582 (2015). find more information here. - tetrazine (MeTet), Absorption and Emission Spectrum (ASCII) CF®450 Dye. B. Dumontel, F. Susa, T. Limongi, V. Vighetto, D. Debellis, M. Canta, V. Cauda, Nanotechnological engineering of extracellular vesicles for the development of actively targeted hybrid nanodevices, Cell & bioscience 12, 61 (2022). H. Mannell, J. Pircher et al., Targeted Endothelial Gene Delivery by Ultrasonic Destruction of Magnetic Microbubbles Carrying Lentiviral Vectors, Pharm. - ATTO 550 absorption/emission (.jpg), Material Safety Data Sheets The fluorescence is excited most efficiently in the range 575 610 nm. 2 Images : +351 30 8808 050 Fax : +351 30 8808 052 info@quimigen.pt www.quimigen.pt Expression of TRPV4 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture.A D. Staining usingAnti-TRPV4Antibody (#ACC-034) (1:500) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.B E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.C. flow cytometry, cancer, Attune NxT. Immunohistochemical staining of rat kidney paraffin embedded region using Anti-Aquaporin 2-ATTO Fluor-550 Antibody (#AQP-002-AO), (1:50), (Red). N. Oleksiievets, C. Mathew, J. Thiele, J. Gallea, O. Nevskyi, I. Gregor, A. Weber, R. Tsukanov, J. Enderlein, Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy, Nano letters 22, 6454 (2022). Warrantee of use applies to the website owners and/or their representative, including the content displayed in the website, as subject to the conditions of use. J. Liu, J. Xue, L. Fu, J. Xu, M. Lord, K. Liang. These percentages are automatically calculated for each compound currently on the graph. D. Bracha, M. Walls, M.-T. Wei, L. Zhu, M. Kurian, J. Avalos, J. Toettcher, C. Brangwynne, Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds, Cell 175, 1467-1480.e13 (2018). Chem. M. Chung, D. Kim, A.E. N. Oleksiievets, C. Mathew, J. Thiele, J. Gallea, O. Nevskyi, I. Gregor, A. Weber, R. Tsukanov, J. Enderlein. Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses. D. Daems, W. Pfeifer, I. Rutten, B. Saccà, D. Spasic, J. Lammertyn, Three-Dimensional DNA Origami as Programmable Anchoring Points for Bioreceptors in Fiber Optic Surface Plasmon Resonance Biosensing, ACS Applied Materials & Interfaces 10, 23539 (2018). Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO) is directly labeled with an ATTO-550 fluorescent dye. 25, 2166 (2014). The choice currently selected will be highlighted in blue. ATTO-488. The BD Special Order Research Product program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Sumita, M.R. Tomov et al., Detailed Study of DNA Hairpin Dynamics Using Single-Molecule Fluorescence Assisted by DNA Origami, J. Phys. Á. Molinero-Fernández, M. Moreno-Guzmán, L. Arruza, M. López, A. Escarpa, Polymer-Based Micromotor Fluorescence Immunoassay for On-the-Move Sensitive Procalcitonin Determination in Very Low Birth Weight Infants’ Plasma, ACS Sensors 5, 1336 (2020). NKaRDW(ob=s*BFnc`9c6 Up to 14 parameters from 4 lasers The PLT-F channel can be selected for testing on any sample or only used as a reflex test if the RBC or platelet size histograms are abnormal or if the platelet count is below a preset limit (determined by the user). Claude, J. Wenger, Fluorescence Brightness, Photostability, and Energy Transfer Enhancement of Immobilized Single Molecules in Zero-Mode Waveguide Nanoapertures, ACS Photonics 9, 2109 (2022). First, we report a robust method for quantifying plasma membrane cholesterol by flow cytometry using the GFP-D4 probe. T` GDbqb~Jh!7}IXc-tOa^ To detect far-red fluorescence in cells labeled with the Alexa Fluor 647 or Cy5 A set of polymer particles stained with at least two fluorescent dyes is presented. ATTO-590. C.R. Cy 5: A standard far red-fluorescent label for nucleic acids used in imaging, flow cytometry, and genomic applications. Kim, W. Sung, N. Lee, Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks, Angewandte Chemie International Edition 54, 8943 (2015). Am. Kim, G. Krainer, D. Lamb, N. Lee, E. Lemke, B. Levesque, M. Levitus, J. McCann, N. Naredi-Rainer, D. Nettels, T. Ngo, R. Qiu, N. Robb, C. Röcker, H. Sanabria, M. Schlierf, T. Schröder, B. Schuler, H. Seidel, L. Streit, J. Thurn, P. Tinnefeld, S. Tyagi, N. Vandenberk, A. Vera, K. Weninger, B. Wünsch, I. Yanez-Orozco, J. Michaelis, C. Seidel, T. Craggs, T. Hugel. J.N. 0000190962 00000 n The fluorescence is excited most efficiently in the range 610 645 nm. 4, 774 (2008). C 114, 4345 (2010). Fluorescent microscopy of human skin tissue section (paraffin fixation) with fungal infection. CF®405L Dye - Biotium K. Banas, N. Rivera-Torres, P. Bialk, B.-C. Yoo, E. Kmiec, Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells, Molecular cancer research : MCR 18, 891 (2020). J. Spitzberg, X. van Kooten, M. Bercovici, A. Meller, Microfluidic device for coupling isotachophoretic sample focusing with nanopore single-molecule sensing, Nanoscale 12, 17805 (2020). 9 0 obj <> endobj ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B, the commercial alternative to NEDTM. M. Malle, P. Löffler, S. Bohr, M. Sletfjerding, N. Risgaard, S. Jensen, M. Zhang, P. Hedegård, S. Vogel, N. Hatzakis. 135, 11935 (2013). Corrie, A. Rühmann, M. Trau, Biomolecular screening with novel organosilica microspheres, Chem. Maximum absorption 554 nm; maximum fluorescence 576 nm. Please activate JavaScript to have access to all shop functions and all shop content. - maleimide Keen, K. Jack et al., A structural study of hybrid organosilica materials for colloid-based DNA biosensors, J. They are analogous to Alexa dyes and are comparable to any fluorescent technology (and used under license from ATTO-TEC). Sung, M.-J. Lyophilized powder. If you ownour legacyBDLSR II Flow Cytometer,you can take advantage of ourexclusive special offers for trading in yourBDLSR IISystem. S. Baliga, C. Murphy, L. Sharon, S. Shenoy, D. Biranthabail, H. Weltman, S. Miller, R. Ramasamy, J. Shah, Rapid method for detecting and differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in sputum by fluorescence in situ hybridization with DNA probes, International Journal of Infectious Diseases 75, 1 (2018). - ATTO 550 carboxy MSDS. It has been designed to recognize TRPV4 from human, rat, and mouse samples. Irving et al., Reactive centre loop mutants of α-1-antitrypsin reveal position-specific effects on intermediate formation along the polymerization pathway, Biosci. ▪ Alexa Fluor 532 ▪ Alexa Fluor 555 ▪ DsRed ▪ Qdot 565 ▪ Qdot 605 ▪ R-phycoerythrin (R-PE, PE), ▪ Qdot 705 ▪ PE-Alexa Fluor 700 ▪ TRI-COLOR (PE-Cy5) ▪ PE-Cy5.5. H. Mönck, D. Toppe, E. Michael, S. Sigrist, V. Richter, D. Hilpert, D. Raccuglia, M. Efetova, M. Schwärzel, A new method to characterize function of the Drosophila heart by means of optical flow, The Journal of experimental biology 220, 4644 (2017). Yang, E. Cooper, B. Chen, K. Siminovitch, A. Peterson, TIE, The CRISPR journal 1, 223 (2018). Am. C. Kimna, O. Lieleg, Engineering an orchestrated release avalanche from hydrogels using DNA-nanotechnology, Journal of Controlled Release 304, 19 (2019). Z. Tang, Q. Wei, A. Wie, Metal-Mesh Litography, Appl. . ✓ Ability to navigate with the keyboard J. Churko, P. Garg, B. Treutlein, M. Venkatasubramanian, H. Wu, J. Lee, Q. Wessells, S.-Y. J. Strmqvist, L. Nardo et al., Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study, Eur. 14, 4707 (2014). This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. L. Dirix, K. Kennes, E. Fron, Z. Debyser, M. van der Auweraer, J. Hofkens, S. Rocha. H. Mönck, D. Toppe, E. Michael, S. Sigrist, V. Richter, D. Hilpert, D. Raccuglia, M. Efetova, M. Schwärzel. View theBD LSRFortessa System brochure. NKaRDW(ob=s*BFnc`9c6 Shan, A protean clamp guides membrane targeting of tail-anchored proteins, Proceedings of the National Academy of Sciences 114, E8585-E8594 (2017). Antibody conjugation is a critical step in many molecular-biology research assays. R. Friedrich, S. Block, M. Alizadehheidari, S. Heider, J. Fritzsche, E. Esbjrner, F. Westerlund, M. Bally, A nano flow cytometer for single lipid vesicle analysis, Lab on a chip 17, 830 (2017). W. Ye, M. Götz, S. Celiksoy, L. Tüting, C. Ratzke, J. Prasad, J. Ricken, S. Wegner, R. Ahijado-Guzmán, T. Hugel, C. Sönnichsen, Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate, Nano letters 18, 6633 (2018). This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs. J. Reyes, S. Ekmark-Léwen, M. Perdiki, T. Klingstedt, A. Hoffmann, E. Wiechec, P. Nilsson, K. Nilsson, I. Alafuzoff, M. Ingelsson, M. Hallbeck, Accumulation of alpha-synuclein within the liver, potential role in the clearance of brain pathology associated with Parkinson's disease, Acta neuropathologica communications 9, 46 (2021). 0000003531 00000 n A (-) in a table cell represents no applicable spillover. ATTO-590. - streptavidin A. Reinhardt, M. Horn et al., Novel Imidazolium Salt−Peptide Conjugates and Their Antimicrobial Activity, Bioconjugate Chem. Chem. Products are lyophilized and ship at room temperature - FREE OF CHARGE. Second, to optically distinguish and quantify intracellular cholesterol accumulation, we have adapted the classical filipin cholesterol staining protocol. B 110, 1976 (2006). Spectrum [Atto 550] - AAT Bioquest 152, 170 (2010). Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates The dye is moderately hydrophilic. Learn More. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. J. Scholefield, R. Henriques, A. Savulescu, E. Fontan, A. Boucharlat, E. Laplantine, A. Smahi, A. Isral, F. Agou, M. Mhlanga, Super-resolution microscopy reveals a preformed NEMO lattice structure that is collapsed in incontinentia pigmenti, Nature Communications 7, 12629 (2016). Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 Antibody (#AAR-011-AO) is directly labeled with an ATTO-550 fluorescent dye. As a control, cells activated with a commercially . Spectra varies slightly from lot to lot. Starbound Weapon Tiers, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, Belongs to the class of Rhodamine dyes. Q. Wang, S. Chear, K. Wing, D. Stellon, M. Nguyen Tran, J. Talbot, A. Pébay, A. Hewitt, A. Cook, Use of CRISPR/Cas ribonucleoproteins for high throughput gene editing of induced pluripotent stem cells, Methods 194, 18 (2021). what special technology allows computers to perform Publish on May 15, 2023 smooth radio london playlist . Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody (#ACC-063-AO) is directly labeled with an ATTO-550 fluorescent dye. Spectra Viewer. Fan, Switching to the cyclic pentose phosphate pathway powers the oxidative burst in activated neutrophils, Nature Metabolism 4, 389 (2022). - ATTO 550 absorption (.txt), Absorption and Emission Spectrum (graphic) S. Chavan, D. Kim, J. Hwang, Y. Choi, J. Hong, J. Kim, M.-H. Lee, M. Hwang, J. Choi, Enhanced Detection of Infectious Pancreatic Necrosis Virus via Lateral Flow Chip and Fluorometric Biosensors Based on Self-Assembled Protein Nanoprobes, ACS Sensors 4, 2937 (2019). 300 400 500 600 700 800 900 Wavelength (nm) CF450. Bioelectr. R. Friedrich, S. Block, M. Alizadehheidari, S. Heider, J. Fritzsche, E. Esbjörner, F. Westerlund, M. Bally, A nano flow cytometer for single lipid vesicle analysis, Lab on a chip 17, 830 (2017). 4, 1000134 (2013). After choosing a subject in the menu, waiting time is necessary for the page to upload. . AQP2 is detected in collecting ducts but not in thin segments of the loop of Henle. E. Britt, J. Lika, M. Giese, T. Schoen, G. Seim, Z. Huang, P. Lee, A. Huttenlocher, J. - phalloidin Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers. 0000191145 00000 n Chen, K. Chetal, G. Mantalas, N. Neff, E. Jabart, A. Sharma, G. Nolan, N. Salomonis, J. Wu. Fluorophore Families - BioLegend Download or Print a poster-sized reagent selection guide, Find reagents for flow cytometry Select fluorophore Find antibodies, For a reagent guide for the first-generation Attune flow cytometer, see Resources for the Original Attune Cytometer. Maximum absorption 630 nm; Maximum fluorescence 651 nm. All rights reserved. The results demonstrate that harmful protein aggregates, or deposits, can bind and hitch a lift with channel-forming proteins, and in this way spread to healthy cells. E. Ronzitti, B. Harke, A. Diaspro, Frequency dependent detection in a STED microscope using modulated excitation light, Optics Express 21, 201 (2013). performed the flow cytometry . Phone: 305-822-0666 CAD cells were . Rinne, T.P. 4, 1000134 (2013). This may be a convenient setting for users employing low-contrast screens. DC sheath flow detection method Sysmex analysers use the DC sheath flow detection method to count red blood cells and platelets, RBC and PLT. Flow cytometry is a technique used to analyze cells for a variety of purposes, including cell counting, phenotyping, cell cycle assessment, and viability. D. Falconnet, J. N. Gilat, D. Torchinsky, S. Margalit, Y. Michaeli, S. Avraham, H. Sharim, N. Elkoshi, C. Levy, S. Zirkin, Y. Ebenstein, Rapid Quantification of Oxidation and UV Induced DNA Damage by Repair Assisted Damage Detection-(Rapid RADD), Analytical Chemistry 92, 9887 (2020). J. Spitzberg, X. van Kooten, M. Bercovici, A. Meller, Microfluidic device for coupling isotachophoretic sample focusing with nanopore single-molecule sensing, Nanoscale 12, 17805 (2020). F. Panzeri, A. Ingargiola et al., Single-molecule FRET experiments with a red-enhanced custom technology SPAD, Proc. Chem. Learn from Jordi Petriz, PhD from Josep Carreras Leukaemia Research Institute about using flow cytometry to detect and analyze immune cells. R. Tsukanov, T.E. - iodoacetamide Chem. Y. Li, J. Bolinger, Y. Yu, Z. - azide/alkyne Gross, C.R. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescent quantum yield, and high photo-stability. introduction Omega Optical. Claude, J. Wenger, Surface passivation of zero-mode waveguide nanostructures, Scientific Reports 10, 1 (2020). Anti-Angiotensin II Receptor Type-1-ATTO Fluor-550 Antibody - Alomone Labs Up to 14 parameters from 4 lasers . Optical data of the carboxy 1 - derivative (in PBS, pH 7.4): ATTO 550 Cy3 abs e max fl h Zhang, J. Lauzadis, S. Liu, S. Shiers, A. Belu, S. Palan, S. Marlin, J. S. Yeou, J. Hwang, J. Yi, C. Kim, S. Kim, N. Lee, Cytosine methylation regulates DNA bendability depending on the curvature, Chemical science 13, 7516 (2022).
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